diff --git a/README.mdown b/README.mdown index 4138c1e..88da293 100644 --- a/README.mdown +++ b/README.mdown @@ -167,7 +167,7 @@ simple sugar | 15 g | 5 g - Optionally add no more than 1 gram of diammonium phosphate (dap) with urea for yeast nutrients. -4. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 10 minutes. +4. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 20 minutes. Allow to cool to room temperature. 5. Replace aluminum foil with mason jar lid, screwing closed tightly to prevent spilling. @@ -214,7 +214,7 @@ malt extract | 6 g | 2 g 5. Fill a sanitary test tube one third (1/3) capacity and replace the screw cap. -6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 10 minutes. +6. Place test tubes in an empty mason jar and [autoclave](#autoclave) for 20 minutes. 7. Carefully remove each test tube and place on a flat surface with the screw cap slightly elevated on a dish towel. @@ -365,10 +365,17 @@ Wood-loving species like oyster and shiitake thrive in a mixture of sawdust. This guide does not address these special requirements. I leave it up to you to research your mycelium species. -[Grain spawn](#grain-spawn) is a common source of nutrients and the most accessible source of grain spawn is wild bird seed. -You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. +Mushroom growers have had some success with the much quicker method of inoculating store bought sterilized whole brown rice. +These are usually sold as -These general instructions are the same for most [grain spawn](#grain-spawn). +> Instant Microwavable Whole Grain Brown Rice + +For your first time I recommend you try this method to learn the process. +Simply cut a small hole in a corner of the sterile container and follow the instructions for [spawn inoculation](#spawn-inoculation). +Tape the small hole closed. + +You should, however, provide mycelium a variety of [grain spawn](#grain-spawn) and [substrates](#bulk-substrate) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. +These general instructions are the same for most [grain spawn](#grain-spawn) but the most accessible source of grain spawn is `wild bird seed`. # Grain Spawn @@ -415,7 +422,7 @@ Imperial | Metric 8. Split evenly to each 16 oz wide mouth mason jar, about 1 cup. -9. Cover un-lid jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours. +9. Cover jar opening with aluminum foil and [autoclave](#autoclave) for 2 hours. 10. Lightly screw lid over the foil to secure it in place without sealing the jar and allow to cool to room temperature. @@ -468,6 +475,9 @@ However, exposing more surface area give the mushrooms more room to sprout. - [ ] take-out containers, black opaque, polypropylene Earlier it was mentioned that mycelium want a variety of [grain spawn](#grain-spawn) and [substrates](https://en.wikipedia.org/wiki/Substrate_(biology)) between generations of colonies so the strain does not lose it's ability to digest different sources of nutrition. + +Once you have practice making [grain spawn](#grain-spawn) you'll want to mix 25% of the substrate chosen for this step to give the mycelium time to produce the right enzymes during colonization. + This guide uses coconut husk (coir) mixed with [grain spawn](#grain-spawn) for colonization as a substrate. You can also add coffee grounds, sawdust, straw, and rice husks for variety. Take caution to only change one thing at a time between generations or you risk overwhelming the mycelium.