removing unessisary utensils

master
JoYo 2020-02-29 17:17:20 -05:00
parent 0f4d0384f8
commit ca870e920e
1 changed files with 17 additions and 26 deletions

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@ -280,18 +280,29 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
## Spore Samples
- [ ] inoculation loop
- [ ] flame or lighter
[Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
These are called spore prints.
Spores can then be added to a syringe of sterile water for ease in shipping.
An inoculation loop is used to apply the spore samples to a [culture medium](#culture-media).
### Spore Syringe
0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter, heat the spore syringe needle until it glows red. Allow to cool.
2. Place a single drop of water from a spore syringe on the [culture medium](#culture-media).
3. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
### Spore Print
An inoculation loop maybe useful to apply the spore print samples to a [culture medium](#culture-media).
Alternatively use a sanitary sharp knife or scalpel to apply the spore print samples.
0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-media).
@ -309,24 +320,8 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
### Spore Syringe
0. [Sanitize Your Workspace](#sanitize-your-workspace).
- Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
2. Place a single drop of water from a spore syringe on the loop.
3. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
4. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
## Wood Plugs
- [ ] tweezers
Mycelium samples are often sold as colonized grain or wood plugs.
Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
Cultures are used to store these samples long-term.
@ -348,11 +343,11 @@ Otherwise, a successful agar slant colony is used to create [liquid culture](#li
- Sanitize the outside of each [culture medium](#culture-media).
1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
1. Using a lighter, heat a sharp knife or scalpel until it glows red. Allow to cool.
2. Gently scrape some of the mycelium off of the surface of agar onto the loop.
2. Carefully cut a small sample of the mycelium colonized agar off.
3. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
3. Insert agar sample into an open [liquid culture](#liquid-cultures) to apply spores.
4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
@ -557,12 +552,8 @@ Harvest mushrooms after their gills are exposed but before they dump their spore
- [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
- [ ] inoculation loop
- [ ] flame or lighter
- [ ] tweezers
- [ ] wild bird seed with millet
- [ ] colander