From ca870e920e1712178da13f9aacca8aa9fddceb60 Mon Sep 17 00:00:00 2001
From: JoYo <>
Date: Sat, 29 Feb 2020 17:17:20 -0500
Subject: [PATCH] removing unessisary utensils

---
 README.mdown | 43 +++++++++++++++++--------------------------
 1 file changed, 17 insertions(+), 26 deletions(-)

diff --git a/README.mdown b/README.mdown
index d317be8..4b563d4 100644
--- a/README.mdown
+++ b/README.mdown
@@ -280,18 +280,29 @@ Piercing the mushroom tissue with a hollow needled syringe is enough to collect
 
 ## Spore Samples
 
-- [ ] inoculation loop
-
 - [ ] flame or lighter
 
 [Spore](https://en.wikipedia.org/wiki/Fungus#Spore_dispersal) samples are created by placing a mushroom cap on a flat surface and collecting the spores that are released.
 These are called spore prints.
 Spores can then be added to a syringe of sterile water for ease in shipping.
 
-An inoculation loop is used to apply the spore samples to a [culture medium](#culture-media).
+### Spore Syringe
+
+0. [Sanitize Your Workspace](#sanitize-your-workspace).
+
+    - Sanitize the outside of each [culture medium](#culture-media).
+
+1. Using a lighter, heat the spore syringe needle until it glows red. Allow to cool.
+
+2. Place a single drop of water from a spore syringe on the [culture medium](#culture-media).
+
+3. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
 
 ### Spore Print
 
+An inoculation loop maybe useful to apply the spore print samples to a [culture medium](#culture-media).
+Alternatively use a sanitary sharp knife or scalpel to apply the spore print samples.
+
 0. [Sanitize Your Workspace](#sanitize-your-workspace).
 
     - Sanitize the outside of each [culture medium](#culture-media).
@@ -309,24 +320,8 @@ An inoculation loop is used to apply the spore samples to a [culture medium](#cu
 
 6. Daily release the pressure within the [culture medium](#culture-media) container by unscrewing the cap.
 
-### Spore Syringe
-
-0. [Sanitize Your Workspace](#sanitize-your-workspace).
-
-    - Sanitize the outside of each [culture medium](#culture-media).
-
-1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
-
-2. Place a single drop of water from a spore syringe on the loop.
-
-3. Insert loop into an open [culture medium](#culture-media) and gently apply spores.
-
-4. Replace the screw cap on the [culture medium](#culture-media) and store in ambient room light at room temperature.
-
 ## Wood Plugs
 
-- [ ] tweezers
-
 Mycelium samples are often sold as colonized grain or wood plugs.
 Usually these can be used to inoculate [grain spawn](#grain-spawn) directly.
 Cultures are used to store these samples long-term.
@@ -348,11 +343,11 @@ Otherwise, a successful agar slant colony is used to create [liquid culture](#li
 
     - Sanitize the outside of each [culture medium](#culture-media).
 
-1. Using a lighter, heat the loop element of your inoculation loop until it glows red. Allow the loop to cool.
+1. Using a lighter, heat a sharp knife or scalpel until it glows red. Allow to cool.
 
-2. Gently scrape some of the mycelium off of the surface of agar onto the loop.
+2. Carefully cut a small sample of the mycelium colonized agar off.
 
-3. Insert loop into an open [liquid culture](#liquid-cultures) and gently apply spores.
+3. Insert agar sample into an open [liquid culture](#liquid-cultures) to apply spores.
 
 4. Replace the screw cap on the [liquid culture](#liquid-cultures) and store in ambient room light at room temperature.
 
@@ -557,12 +552,8 @@ Harvest mushrooms after their gills are exposed but before they dump their spore
 
 - [ ] 30 to 50 ml test tubes with screw cap, [autoclavable](#autoclave)
 
-- [ ] inoculation loop
-
 - [ ] flame or lighter
 
-- [ ] tweezers
-
 - [ ] wild bird seed with millet
 
 - [ ] colander