note on colonizing grain spawn without mixing in substrate.

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JoYo 2022-01-31 14:10:22 -05:00
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# HomeMyco # HomeMyco
A [homebrewer](https://en.wikipedia.org/wiki/Homebrewing)'s take on practical home cultivation of [mycelium](https://en.wikipedia.org/wiki/Mycelium) and their [mushrooms](https://en.wikipedia.org/wiki/Mushroom). A [homebrewer](https://en.wikipedia.org/wiki/Homebrewing)'s take on practical home cultivation of [mycelium](https://en.wikipedia.org/wiki/Mycelium) and their [mushrooms](https://en.wikipedia.org/wiki/Mushroom).
Mycelium are kind of like a mushroom's roots but this is not an exact analogy. Mycelium are like a mushroom's roots but this is not an exact analogy.
The purpose of a mushroom is to release spores as far away from the colony as it can reach. The use of a mushroom to the mycelium is to release spores as far away from the colony as it can reach.
[Creative Commons Attribution-NonCommercial-ShareAlike 4.0](LICENSE) [Creative Commons Attribution-NonCommercial-ShareAlike 4.0](LICENSE)
@ -12,7 +12,7 @@ Contamination occurs when other microorganisms take advantage of the gratuity yo
There is always a risk that bacteria and mold will invade, competing with your precious mushroom makers. There is always a risk that bacteria and mold will invade, competing with your precious mushroom makers.
Many guides do everything they can to reduce this risk. Many guides do everything they can to reduce this risk.
My approach gives you methods that are still effective but easy and cheap to recover from contamination. My approach gives you methods that are also effective but easy and cheap to recover from contamination.
# Do it Thrice # Do it Thrice
@ -27,7 +27,7 @@ As a result of the above hubris we triple our efforts.
Bird seed, coconut husk, and sugar water are inexpensive. Bird seed, coconut husk, and sugar water are inexpensive.
All other equipment can be reused. All other equipment can be reused.
Saving a control will help you test for contamination introduced through your technique. Saving a control will help you test for contamination introduced through your technique.
If all fares well you can always using it the next time you do inoculations. You can always use the control the next time you do inoculations.
# Finding Samples # Finding Samples
@ -44,7 +44,7 @@ There remains the risk that grocery store mushrooms are contaminated.
Online shopping can get you all sorts of mycelium sample types, ranging from spawn grains and liquid culture to spores and dried mushrooms. Online shopping can get you all sorts of mycelium sample types, ranging from spawn grains and liquid culture to spores and dried mushrooms.
When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate. When paying good money for a sample try to find liquid culture as they have a pretty good colonization rate.
Eventually you will want to make your own cultures from your harvested mushrooms. Eventually you may want to make your own cultures from your harvested mushrooms.
# Three Stages # Three Stages
@ -304,6 +304,7 @@ These general instructions are the same for most [grain spawn](#grain-spawn-and-
Nutrient rich grain treated and sanitized to feed mycelium. Nutrient rich grain treated and sanitized to feed mycelium.
Vitamin and mineral rich substrate treated and sanitized to provide mycelium a growing surface. Vitamin and mineral rich substrate treated and sanitized to provide mycelium a growing surface.
In this guide we combine grain spawn and substrate to simplify the process and reduce risk of contamination.
- [ ] 16 oz wide mouth mason jars with lids - [ ] 16 oz wide mouth mason jars with lids
@ -343,7 +344,7 @@ These endophytes do not protect our mushroom roots and will compete for resource
0. Fill with water until moisture is visible on the surface of the mixture and soak overnight. 0. Fill with water until moisture is visible on the surface of the mixture and soak overnight.
0. Split evenly to each 16 oz wide mouth mason jar, about 1 cup. 0. Split evenly to each 16 oz wide mouth mason jar no further than the 300 ml mark.
0. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 2 hours. 0. Cover jar opening with aluminum foil and [pressure cook](#autoclave) for 2 hours.
@ -351,6 +352,10 @@ These endophytes do not protect our mushroom roots and will compete for resource
0. Store in a dark cool place until inoculation. 0. Store in a dark cool place until inoculation.
When you become more familiar with the above process you may wish to colonize only grain spawn prior to mixing with substrate.
This process is easier to view contamination and to agitate the mycelium to increase the colonization rate.
Once grain spawn is fully colonize you would mix the colonized grain spawn with the substrate to provide adequate fruiting space.
## Spawn Inoculation ## Spawn Inoculation
- [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave) - [ ] 50 to 100 ml syringe with hollow needle, [autoclavable](#autoclave)